Thông tin chi tiết tài liệu

Quốc hội

Thủ tướng Chính phủ

Liên bộ

Danh sách các Bộ, Ngành

Click vào tìm kiếm

Danh sách các tỉnh & thành phố trực thuộc Trung ương

Chi tiết tài liệu

Biochemical Roles of Eukaryotic Cell Surface Macromolecules

Tác giả: Chakrabarti, Abhijit; Surolia, Avadhesha

Chủ đề:

Chủ đề: Medicine

Nhà xuất bản: Springer

Năm xuất bản: 2015

Loại tài liệu: Book

Mô tả vật lý: 406 p.

Định danh: 978-3-319-11280-0

Ngôn ngữ: en

Liên kết: Advances in Experimental Medicine and Biology;Volume 824

Đọc trực tuyến

Tải tài liệu

Consists of critical reviews and original research papers from the 2014 International Symposium on the "Biochemical Role of Eukaryotic Cell Surface Macromolecules". Topics covered include: ã neurochemical and biochemical analysis of cell surface glycoconjugates ã membrane skeletal organization ã GPCRs and other receptors ã biophysical approaches to study membrane interactions ã glycoconjugate metabolism ã dysregulation ã molecular mechanisms involved in cell-cell and cell-matrix interaction ã glycans in infectious and neurological diseases ã cancer and glycosyltransferases as drug targets.

Chapter 24: Structural Heterogeneity of Glycoform of Alpha-1 Acid Glycoprotein in Alcoholic Cirrhosis Patients
- Discussion
- Introduction
- Materials and Methods
  - Chemicals and HPLC Columns
  - ELISA and Lectin Blotting
  - HPLC Separation of PA-glycans
  - Patients
  - Purification of Alpha-1-acid Glycoprotein and Preparation of Fluorescent Labeled N-glycans
- References
- Results
Index
Cover
Advances in Experimental Medicine and Biology
Title pages
Preface
Contents
Chapter 1: Human-Specific Evolutionary Changes in the Biology of Siglecs
- Introduction
- Human-Specific Changes in Siglecs
  - Siglec-1/Sialoadhesin
  - Siglec-3/CD33
  - Siglec-5 and -14
  - Siglec-6
  - Siglec-9
  - Siglec-11 and -16
  - Siglec-XII
  - Siglec-13
  - Siglec-17
- Conclusions and Perspectives
- References
Chapter 2: Structural Changes of GPI Anchor After Its Attachment to Proteins: Functional Significance
- Introduction
- Biosynthesis of GPI and Its Attachment to Proteins
- GPI Remodeling Reactions That Occur After Attachment to Proteins
  - Inositol-Deacylation by PGAP1 in the ER
  - Removal of an EtNP Side Branch from the Second Mannose by PGAP5 in the ER
  - Fatty Acid Remodeling of GPI-AP in the Golgi
  - Biological Significance of Fatty Acid Remodeling
- References
Chapter 3: Novel Insights in Membrane Biology Utilizing Fluorescence Recovery After Photobleaching
- Fluorescence Recovery After Photobleaching
- The Dynamic Membrane
- Lipid Dynamics by FRAP
- GPCR Activation: Manifestations in Receptor Dynamics
- Dynamic Confinement of GPCRs Upon Cholesterol Depletion: Insight from Bleach Area-Dependent FRAP
- Are Signaling and Dynamics Correlated?
- Conclusion and Future Perspectives
- Lateral Dynamics as Readout of Infection
- References
Chapter 4: Defects in Erythrocyte Membrane Skeletal Architecture
- Introduction
- Membrane Skeleton
  - Erythroid Membrane Skeleton
  - Lipid Bilayer: Membrane Skeleton Interactions
  - Assembly of the Membrane-Skeleton of Erythroid Cells
- Defects in Membrane Skeleton
  - Hereditary Spherocytosis
    - Molecular Defects in HS
    - Pathophysiology
    - Laboratory Features and Treatment
  - Hereditary Elliptocytosis
    - Molecular Defects in HE
    - Laboratory Features and Treatment
  - Malaria and the Erythrocyte Membrane Skeleton Alterations
- Conclusion
- References
Chapter 5: Membrane Rafts in the Erythrocyte Membrane: A Novel Role of MPP1p55
- Introduction
- Erythrocyte Membrane Rafts
- Stomatin, Flotillins and MPP1 Are the Stable Components of Erythrocyte Rafts
  - Stomatin
  - Flotillins
  - MPP1
- Visualization of Erythrocyte Membrane Rafts
- Role of Erythrocyte Membrane Rafts in Signaling
- Erythrocyte Rafts and Pathology
- MPP1 and Its Palmitoylation Play a Crucial Role in Lateral Membrane Organization in Erythroid Cells
- Conclusions
- The Involvement of Actin in Erythrocyte Membrane Rafts Organization?
- References
Chapter 6: Immuno-Modulatory Role of Porins: Host Immune Responses, Signaling Mechanisms and Vaccine Potential
- Introduction
- Role of Porins in Modulation of Immune Responses
  - Innate Immune Responses
  - Adaptive Immune Response
- Signaling Cascades Initiated by Porins
- Porins as Vaccine Candidates
- Conclusion
- References
Chapter 7: Vibrio cholerae Cytolysin: Structure–Function Mechanism of an Atypical β-Barrel Pore-ưForming Toxin
- Introduction
- Vibrio cholerae Cytolysin
- Structural Features of VCC
- Mechanism of Membrane Pore Formation
- Structural Features of the VCC β-Barrel Pore
- Conclusion
- References
Chapter 8: New Vis-Tas in Lactosylceramide Research
- Introduction
- Inflammation and the LacCer: Phospholipase-A-2 (PLA2) Connection
- Implications of LacCer Modulation on Cardiac Hypertrophy
- Perspectives
- References
Chapter 9: Plasma Membrane-Associated Sialidase Confers Cancer Initiation, Promotion and Progression
- Introduction
- Upregulation of NEU3 Promotes Cancer Progression
- NEU3 Is Involved in Cancer Initiation and Promotion
- Conclusion
- References
Chapter 10: A Signal with a Difference: The Role of GPI Anchor Signal Sequence in Dictating Conformation and Function of the Als5 Adhesin in Candida albicans
- Introduction
  - GPI Anchors in C. albicans
  - The Role of C-Terminal Signal Sequences in the Attachment of the Anchor
  - Mutations in SS
- Effect of Mutations on the Conformation and Function of Als5-SS
  - Identifying Mutations in Als5-SS That Would Abrogate β-Aggregation Potential of Putative Interacting Domains
  - In silico Predictions of GPI Anchor Attachment for Als5-SS and Its Mutant Variants
  - Generating Als5-SS Variants Carrying the Different Mutations
  - The Als5-SS Mutant Variants Had a Conformation That Was Different from That of Als5-SS
  - The Als5-SS Mutant Variants Adhere Better to Collagen Type IV than Als5-SS Itself
  - The Als5-SS Mutant Variants Show Much Better Aggregation That Wild Type Als5-SS
  - Mutations in a Synthetic SS Peptide Prevent It from Inhibiting Self-Aggregation of Als5
- Concluding Remarks
- References
Chapter 11: Novel Chondroitin Sulfate Oligosaccharide Motifs as Biomarkers: Insights into Their Involvement in Brain Development
- Developmental Changes in the Structures of CS Chains in the Brain
- Introduction
- Epitope Oligosaccharide Sequences of mAbs That Recognize Brain CS Chains
- Isolation of Functional Domain Oligosaccharides from Embryonic Pig Brains
- Neurite Outgrowth-Promoting Activity of Oversulfated CS Chains
- Isolation of Functional Domain Oligosaccharides from the CS-DS Hybrid Chains Purified from Embryonic Pig Brains
- Mechanism Underlying the Neurite Outgrowth-Promoting Activities of CS Chains
- Novel Highly Sulfated Hexasaccharide Sequences Isolated from CS Derived from Shark Fin Cartilage
- Importance of the Three-Dimensional Structure and Electrostatic Potential Distribution at the Molecular Surfaces of Functional Oligosaccharides
- Perspectives
- References
Chapter 12: Role of Hyaluronidases in the Catabolism of Chondroitin Sulfate
- Introduction
- Chn/CS-Specific Hydrolase
- Cellular Localization of Hyal4
- Systemic Catabolism of CS
- Measurement of HYAL1 Activity Toward CS Variants
- Distribution of Glycosaminoglycans in the Animal Kingdom
- Evidence for the Involvement of HYAL1 in the Systemic Degradation of CS
- Perspectives
- References
Chapter 13: Pattern Recognition in Legume Lectins to Extrapolate Amino Acid Variability to Sugar Specificity
- Introduction
- Methodology
- Results and Discussion
  - (Dis)similarites in Legume Lectins Based on Percentage Identities
  - Amino Acid Variability in Legume Lectins Based on Percentage Composition
- Conclusion
- References
Chapter 14: Conformational Dynamics of Oligosaccharides Characterized by Paramagnetism-Assisted NMR Spectroscopy in Conjunction with Molecular Dynamics Simulation
- Introduction: Static and Dynamic Views of Carbohydrate–Protein Interactions
- How to Prepare NMR Samples
- How to Obtain Atomic Long-Distance Information
- How to Deal with Dynamic Conformational Ensembles
- Concluding Remarks and Perspectives: Glycolipid clusters as a Platform for Protein Interactions
- References
Chapter 15: Characterization of Cholesterol Crystalline Domains in Model and Biological Membranes Using X-Ray Diffraction
- Introduction
- Lipid Rafts
- Membrane Effects of Cholesterol Enrichment
- Cholesterol Domains in Model and Biological Membranes
- Membrane Structural Analysis Using Small Angle X-ray Diffraction
- Role of Cholesterol Domains in Membrane Function
- X-Ray Diffraction Method
- Cholesterol Domains in Vascular Smooth Muscle Cell Membranes
- Cholesterol Domains in Model Membranes Exposed to Atherogenic Conditions
- Cholesterol Domains in Ocular Lens Fiber Cell Membranes
- Conclusion
- References
Chapter 16: Role of Lipid-Mediated Effects in β2-ưAdrenergic Receptor Dimerization
- Introduction
- Methods
- Results
  - Indirect Effects: Hydrophobic Mismatch Around the Receptor
  - Direct Effects: Specific Protein-Lipid Interactions
  - Characterization of the Lipid Site at Helix I
  - Exploring the Energetics of Association
- Discussion
- References
Chapter 17: Effect of Temperature on the Phase Behaviour of Fully Saturated DAPC Lipid Bilayer: A Comparative Molecular Dynamics Simulation Study
- Introduction
- Methodology
  - Simulation Protocol
  - System Setup
- Area Per Lipid
- Results and Discussion
- Atom Density Distribution and Lipid Hydrophobic Thickness
- Order Parameter
- Headgroup Orientation of Lipids
- Rotational Autocorrelation Function
- Energy
- Interaction with Water
- Conclusion
- References
Chapter 18: Biophysical Characterization of the Interaction of O-acylcholines with the Major Bovine Seminal Plasma Protein, PDC-109
- Introduction
- Materials and Methods
  - Materials
  - Fluorescence Spectroscopy
  - Isothermal Titration Calorimetry (ITC)
  - Circular Dichroism Spectroscopy
  - Differential Scanning Calorimetry
- Results
  - Isothermal Titration Calorimetry
  - Fluorescence Studies on the Interaction of PDC-109 with O-lauroylcholine
  - CD Spectroscopy
  - Differential Scanning Calorimetry
- Discussion
- References
Chapter 19: Crystal Structure of Apo and Ligand Bound Vibrio cholerae Ribokinase (Vc-RK): Role of Monovalent Cation Induced Activation and Structural Flexibility in Sugar Phosphorylation
- Introduction
- Materials and Methods
  - Cloning, Expression and Purification
  - Crystallization and Data Collection
  - Structural Analysis
  - Structure Determination and Refinement
- Results
  - Overall Structure
  - Dimerization
  - Half-Way Closed Substrate Dimer
  - Open Apo Dimer
  - The Active Site
    - Ribose Binding
    - Monovalent Cation Binding
- Discussion
- References
Chapter 20: Synthetic Glycolipids and (p)ppGpp Analogs: Development of Inhibitors for Mycobacterial Growth, Biofilm and Stringent Response
- Introduction
- Mycobacterial Cell Wall
- Biofilm
- Biofilm and Human Pathogens
- Synthetic Glycolipids—Potential Inhibitors of Biofilm Formation
- Sliding Motility
- Growth Phases of Bacteria and Inhibitors
- Stringent Response and Persistence
- GMP: Intrinsic Regulator of pppGpp Synthesis
- Cyclic-di-GMP
- Conclusion
- References
Chapter 21: Regulations of Glycolipid: XI. Glycosyltransferase (GSL: GLTs) Genes Involved in SA-LeX and Related GSLs Biosynthesis in Carcinoma Cells by Biosimilar Apoptotic Agents: Potential Anticancer Drugs
- Introduction
  - Biosimilar Drugs in Cancer Chemotherapy
  - Lewis Antigen Epitopes on Cancer Cell Surfaces
  - Induction of Apoptosis in Cancer Cells
- Induction of Apoptosis in Breast and Colon Carcinoma Cells by Simple Apoptotic Agents
- Betulin and Betulinic Acid as Apoptotic Agents in Cancer Cells
- Disialogangliosides
- Proteins and Peptides
- Apoptotic Membrane Damages Monitored by Fluorescence Microscopic Studies
  - Fluorescence Staining of Apoptotic Carcinoma Cells
  - PSS-380/or AKS-0 Staining
  - PSS-380/Propidium Iodide (PropI) Staining
  - Image Capture and Processing
- Biosynthesis of Lewis and Blood Group Active Glycosphingolipids in Carcinoma Cells
- Regulation of Lewis Glycosphingolipid (LeX, SA-LeX) Biosynthetic Genes in Normal Tissues and Apoptotic Breast Cancer Cells
- Novel Drug Delivery Systems for Cancer Treatments
- References
- Summary
Chapter 22: N-Acetylglucosaminyl 1-Phosphate Transferase: An Excellent Target for Developing New Generation Breast Cancer Therapeutic
- Introduction
- Results and Discussion
- Conclusion and Future Direction
- References
Chapter 23: Involvement of Vascular Endothelial Growth Factor in Serotonin 1A Receptor-Mediated Neuroproliferation in Neonatal Mouse Hippocampus
- Introduction
- Materials and Method
- Results
- Discussion
- References